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anti ephrinb3  (R&D Systems)


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    R&D Systems anti ephrinb3
    Anti Ephrinb3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+ephrinb3/Human+Ephrin-B3+Antibody/pm31011859-103-44-49
    Average 91 stars, based on 8 article reviews
    anti ephrinb3 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Expressing:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Western Blot:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Labeling:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Derivative Assay:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Binding Assay:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Control:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Hybridization:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Recombinant:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Injection:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Immunostaining:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Isolation:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Staining:

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/ - mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury
    Article Snippet: Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.Tissue extracts were added to sample buffer containing SDS/β-mercaptoethanol and loaded onto 7.5% acrylamide gels for electrophoresis, with each lane containing the equivalent of protein from two optic nerves or from one retina.. After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).. Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).Immunoreactivity was detected using donkey anti-goat antibodies conjugated to peroxidase (1:50,000; Jackson ImmunoResearch) and visualized using SuperSignal West Pico Chemiluminescent (Pierce, Rockford, IL).



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    R&D Systems Hematology goat anti human ephrinb3
    Fig. 6 <t>EphrinB3</t> improves SC migration on FN-coated surfaces. a, b Exit of SC entrapped in an agarose drop and seeded on FN + Fc (a) and FN + EphrinB3 (b), scale bar 200 µm. (c) More SC exit the agarose drop 5 h post-seeding on FN + EphrinB3 than on con- trol surface (two-way ANOVA with repeated measures: p = 0.03, F(1,4) = 10.24). d SC migrate over longer distances from the drop-edge 4 h post- seeding (two-way ANOVA with repeated measures: p = 0.02, F(1,4) = 12.74) on FN + EphrinB3-coated surfaces (n = 3 per group). Graphs represent the values of separate experiments (mean ± SD). e Velocity of single SC was measured in different condi- tions. Only SC seeded on FN + EphrinB3 show a sig- nificant increase of migration speed. This increase is reverted when SC are pre-incubated with anti-EphA4 (one-way ANOVA p = 0.002, F(5,93) = 4.1; two-tailed Mann–Whitney test p = 0.002 between control FN + Fc and FN + EphrinB3 group, n = 12–18 per group). In e, n represents tracked single cells of three different experi- ments repeated independently. Data are expressed as mean values ± SD. Single cells were tracked from three different independent experiments, **p < 0.01
    Goat Anti Human Ephrinb3, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 4. Expression of EphB3 protein in the injured adult optic nerve and macrophages. A, Immunoblots of protein prepara- tionsfromuninjured(UI)andinjuredadultopticnerves8dafterinjury(8)probedusingananti-EphB3antibody.Asignalat110 kDacorrespondingtotheexpectedsizeforEphB3proteinwasdetectedinbothsamples.Thisproteinbandwasnotpresentinoptic nerve tissue obtained from EphB3 null animals (KO). GAPDH-immunopositive bands used as protein loading controls are shown below.B,ED1-positivemacrophagesisolatedfromsegmentsofinjuredadultopticnerveinculture.C,Themacrophagesshownin B bound exogenously applied <t>EphrinB3-Fc</t> protein resulting in a punctate–aggregate pattern of cell-surface labeling. D, ED1- positive macrophages from segments of injured adult optic nerve in culture derived from an EphB3 homozygous null animal. E, ExogenouslyappliedEphrinB3-FcproteinfailedtobindtotheEphB3nullmacrophagesshowninD.F,ED1-positivemacrophages inanadultopticnerve5dafterinjury(green).G,EphrinB3-FcbindinginthesameregionshowninD(red).H,ColocalizationofED1 immunoreactivity with EphrinB3-Fc binding. Scale bars: (in B) B–E, 20 m; (in F) F–H, 10 m.
    Anti Human Ephrinb3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+ephrinb3/Human+Ephrin-B3+Antibody/10__1523_slash_jneurosci__4797___05__2006-81-25-28
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    Image Search Results


    Fig. 6 EphrinB3 improves SC migration on FN-coated surfaces. a, b Exit of SC entrapped in an agarose drop and seeded on FN + Fc (a) and FN + EphrinB3 (b), scale bar 200 µm. (c) More SC exit the agarose drop 5 h post-seeding on FN + EphrinB3 than on con- trol surface (two-way ANOVA with repeated measures: p = 0.03, F(1,4) = 10.24). d SC migrate over longer distances from the drop-edge 4 h post- seeding (two-way ANOVA with repeated measures: p = 0.02, F(1,4) = 12.74) on FN + EphrinB3-coated surfaces (n = 3 per group). Graphs represent the values of separate experiments (mean ± SD). e Velocity of single SC was measured in different condi- tions. Only SC seeded on FN + EphrinB3 show a sig- nificant increase of migration speed. This increase is reverted when SC are pre-incubated with anti-EphA4 (one-way ANOVA p = 0.002, F(5,93) = 4.1; two-tailed Mann–Whitney test p = 0.002 between control FN + Fc and FN + EphrinB3 group, n = 12–18 per group). In e, n represents tracked single cells of three different experi- ments repeated independently. Data are expressed as mean values ± SD. Single cells were tracked from three different independent experiments, **p < 0.01

    Journal: Acta neuropathologica

    Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

    doi: 10.1007/s00401-019-02011-1

    Figure Lengend Snippet: Fig. 6 EphrinB3 improves SC migration on FN-coated surfaces. a, b Exit of SC entrapped in an agarose drop and seeded on FN + Fc (a) and FN + EphrinB3 (b), scale bar 200 µm. (c) More SC exit the agarose drop 5 h post-seeding on FN + EphrinB3 than on con- trol surface (two-way ANOVA with repeated measures: p = 0.03, F(1,4) = 10.24). d SC migrate over longer distances from the drop-edge 4 h post- seeding (two-way ANOVA with repeated measures: p = 0.02, F(1,4) = 12.74) on FN + EphrinB3-coated surfaces (n = 3 per group). Graphs represent the values of separate experiments (mean ± SD). e Velocity of single SC was measured in different condi- tions. Only SC seeded on FN + EphrinB3 show a sig- nificant increase of migration speed. This increase is reverted when SC are pre-incubated with anti-EphA4 (one-way ANOVA p = 0.002, F(5,93) = 4.1; two-tailed Mann–Whitney test p = 0.002 between control FN + Fc and FN + EphrinB3 group, n = 12–18 per group). In e, n represents tracked single cells of three different experi- ments repeated independently. Data are expressed as mean values ± SD. Single cells were tracked from three different independent experiments, **p < 0.01

    Article Snippet: Neutralization of EphrinB3 epitopes in myelin extracts EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology, ratio: 1:1) for 2 h at room temperature prior to the addition to the cells [55].

    Techniques: Migration, Incubation, Two Tailed Test, MANN-WHITNEY, Control

    Fig. 8 Model of the mechanism of guidance and migration of SC after CNS demyelination. a, b SC encountering CNS white matter are activated by the myelin-associated EphrinB3 through EphB6- and EphA4-SC receptors. c, d The activation of these receptors by phospho- rylation impairs SC adhesion to white matter and increases SC expression of Integrinβ1, promoting their adhesion to BV extracellular matrix. e Lesions of white matter undergo the formation and/or remodeling of BV which increases expression of ECM adhesion molecules, such as FN, and further facili- tate SC mobilization towards the lesion

    Journal: Acta neuropathologica

    Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

    doi: 10.1007/s00401-019-02011-1

    Figure Lengend Snippet: Fig. 8 Model of the mechanism of guidance and migration of SC after CNS demyelination. a, b SC encountering CNS white matter are activated by the myelin-associated EphrinB3 through EphB6- and EphA4-SC receptors. c, d The activation of these receptors by phospho- rylation impairs SC adhesion to white matter and increases SC expression of Integrinβ1, promoting their adhesion to BV extracellular matrix. e Lesions of white matter undergo the formation and/or remodeling of BV which increases expression of ECM adhesion molecules, such as FN, and further facili- tate SC mobilization towards the lesion

    Article Snippet: Neutralization of EphrinB3 epitopes in myelin extracts EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology, ratio: 1:1) for 2 h at room temperature prior to the addition to the cells [55].

    Techniques: Migration, Activation Assay, Expressing

    Figure 4. Expression of EphB3 protein in the injured adult optic nerve and macrophages. A, Immunoblots of protein prepara- tionsfromuninjured(UI)andinjuredadultopticnerves8dafterinjury(8)probedusingananti-EphB3antibody.Asignalat110 kDacorrespondingtotheexpectedsizeforEphB3proteinwasdetectedinbothsamples.Thisproteinbandwasnotpresentinoptic nerve tissue obtained from EphB3 null animals (KO). GAPDH-immunopositive bands used as protein loading controls are shown below.B,ED1-positivemacrophagesisolatedfromsegmentsofinjuredadultopticnerveinculture.C,Themacrophagesshownin B bound exogenously applied EphrinB3-Fc protein resulting in a punctate–aggregate pattern of cell-surface labeling. D, ED1- positive macrophages from segments of injured adult optic nerve in culture derived from an EphB3 homozygous null animal. E, ExogenouslyappliedEphrinB3-FcproteinfailedtobindtotheEphB3nullmacrophagesshowninD.F,ED1-positivemacrophages inanadultopticnerve5dafterinjury(green).G,EphrinB3-FcbindinginthesameregionshowninD(red).H,ColocalizationofED1 immunoreactivity with EphrinB3-Fc binding. Scale bars: (in B) B–E, 20 m; (in F) F–H, 10 m.

    Journal: Journal of Neuroscience

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury

    doi: 10.1523/jneurosci.4797-05.2006

    Figure Lengend Snippet: Figure 4. Expression of EphB3 protein in the injured adult optic nerve and macrophages. A, Immunoblots of protein prepara- tionsfromuninjured(UI)andinjuredadultopticnerves8dafterinjury(8)probedusingananti-EphB3antibody.Asignalat110 kDacorrespondingtotheexpectedsizeforEphB3proteinwasdetectedinbothsamples.Thisproteinbandwasnotpresentinoptic nerve tissue obtained from EphB3 null animals (KO). GAPDH-immunopositive bands used as protein loading controls are shown below.B,ED1-positivemacrophagesisolatedfromsegmentsofinjuredadultopticnerveinculture.C,Themacrophagesshownin B bound exogenously applied EphrinB3-Fc protein resulting in a punctate–aggregate pattern of cell-surface labeling. D, ED1- positive macrophages from segments of injured adult optic nerve in culture derived from an EphB3 homozygous null animal. E, ExogenouslyappliedEphrinB3-FcproteinfailedtobindtotheEphB3nullmacrophagesshowninD.F,ED1-positivemacrophages inanadultopticnerve5dafterinjury(green).G,EphrinB3-FcbindinginthesameregionshowninD(red).H,ColocalizationofED1 immunoreactivity with EphrinB3-Fc binding. Scale bars: (in B) B–E, 20 m; (in F) F–H, 10 m.

    Article Snippet: After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).

    Techniques: Expressing, Western Blot, Labeling, Derivative Assay, Binding Assay

    Figure 5. EphrinB3 mRNA and protein expression in the adult retina and on RGC axons. A, EphrinB3 mRNA expression in the ganglion cell layer and the inner nuclear layer of the retina from a normaladultmouse.[SeeDforlocationofganglioncelllayer(GCL)andinnernuclearlayer(INL).]B,EphrinB3mRNAexpressionintheganglioncelllayerandtheinnernuclearlayer4dafteroptic nerveinjury.C,EphrinB3mRNAexpressionintheganglioncelllayerandtheinnernuclearlayer12dafteropticnerveinjury.D,RetinalsectionfromanormaladultmouseexposedtotheEphrinB3 sense control probe. E, EphrinB3 mRNA expression in the optic nerve of a normal adult mouse. A faint hybridization signal is detected in cells distributed in a row-like manner. F, EphrinB3 mRNA expression in the optic nerve of an adult mouse 4 d after optic nerve injury. G, EphrinB3 mRNA expression in the optic nerve of an adult mouse 12 d after optic nerve injury. H, Anti-EphrinB3 immunoblot.Afaintsignalisdetectedintheproteinsamplefromuninjuredopticnerves(UI).Abandat65kDa,theexpectedsizeofEphrinB3,ispresentintheopticnervesamples8dafterinjury (8).I,Uninjuredopticnervesectionafterstainingusinganti-EphrinB3antibody.ThereisdiffusestainingparticularlyattheONHregion.J,Opticnerve(ON)tissuesection12dafterinjurystainedwith anti-EphrinB3antibody.Astrongsignalwasdetectedintheopticnerveproximaltotheinjurysite.CT,Connectivetissue;L,lesionsite.K,SectionfromthesameopticnerveshowninJafterstaining with a secondary antibody alone. L, Recombinant EphB3-Fc protein injected into the adult optic nerve binds to RGC axons. EphB3-Fc was detected using an anti-Fc antibody. M, Fc control protein injected into the adult optic nerve is distributed as diffuse punctate aggregates and does not result in apparent RGC axon binding. Scale bars: A–G, I–K, 100 m; L, M, 20 m.

    Journal: Journal of Neuroscience

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury

    doi: 10.1523/jneurosci.4797-05.2006

    Figure Lengend Snippet: Figure 5. EphrinB3 mRNA and protein expression in the adult retina and on RGC axons. A, EphrinB3 mRNA expression in the ganglion cell layer and the inner nuclear layer of the retina from a normaladultmouse.[SeeDforlocationofganglioncelllayer(GCL)andinnernuclearlayer(INL).]B,EphrinB3mRNAexpressionintheganglioncelllayerandtheinnernuclearlayer4dafteroptic nerveinjury.C,EphrinB3mRNAexpressionintheganglioncelllayerandtheinnernuclearlayer12dafteropticnerveinjury.D,RetinalsectionfromanormaladultmouseexposedtotheEphrinB3 sense control probe. E, EphrinB3 mRNA expression in the optic nerve of a normal adult mouse. A faint hybridization signal is detected in cells distributed in a row-like manner. F, EphrinB3 mRNA expression in the optic nerve of an adult mouse 4 d after optic nerve injury. G, EphrinB3 mRNA expression in the optic nerve of an adult mouse 12 d after optic nerve injury. H, Anti-EphrinB3 immunoblot.Afaintsignalisdetectedintheproteinsamplefromuninjuredopticnerves(UI).Abandat65kDa,theexpectedsizeofEphrinB3,ispresentintheopticnervesamples8dafterinjury (8).I,Uninjuredopticnervesectionafterstainingusinganti-EphrinB3antibody.ThereisdiffusestainingparticularlyattheONHregion.J,Opticnerve(ON)tissuesection12dafterinjurystainedwith anti-EphrinB3antibody.Astrongsignalwasdetectedintheopticnerveproximaltotheinjurysite.CT,Connectivetissue;L,lesionsite.K,SectionfromthesameopticnerveshowninJafterstaining with a secondary antibody alone. L, Recombinant EphB3-Fc protein injected into the adult optic nerve binds to RGC axons. EphB3-Fc was detected using an anti-Fc antibody. M, Fc control protein injected into the adult optic nerve is distributed as diffuse punctate aggregates and does not result in apparent RGC axon binding. Scale bars: A–G, I–K, 100 m; L, M, 20 m.

    Article Snippet: After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).

    Techniques: Expressing, Control, Hybridization, Western Blot, Recombinant, Injection, Binding Assay

    Figure 6. EphB3-Fc protein supports adult RGC axon outgrowth. A, Adult retinal ex- plant grown on a substratum coated with low levels of laminin (2.5 g/ml). This level of laminincoatingsupportsonlyminimalaxonoutgrowth(arrowpointstoanaxon).B,Adult retinal explant grown for 3 d on a substratum coated with low laminin (2.5 g/ml) and EphB3-Fc (9 g/ml). The presence of EphB3-Fc increased the amount of axon outgrowth from adult retinal explants. C, Growth cone extending on low laminin (2.5 g/ml) and EphB3-Fc (9 g/ml). D, Growth cone extending on L1 (0.25 g/ml) and EphB3-Fc (10 g/ml).E,Anti-tubulinstaininginagrowthconesimilarto C.F,Phalloidinstaininginthe same growth cone as in E. G, Anti-GAP-43 immunostaining in a retinal axon grown on a laminin substratum (10 g/ml). H, Immunostaining showing L1 protein expression on RGC axons within the optic nerve 12 d after injury. The asterisk indicates the injury site. I, Graph showing the dose–response relationship between the mean axon number with increasing concentrations of EphB3-Fc protein used for coating onto a laminin substra- tum.Thenumbersatthetopofeachcolumnindicatethenumberofexplantsexaminedfor the given experimental condition. J, Graph showing the relationship between total axon length (mean SEM) with increasing concentrations of EphB3-Fc protein used for sub- stratum coating. The numbers at the top of each column indicate the number of explants examined for the given experimental condition. K, Graph showing the dose–response relationship between the percentage of retinal explants with axon outgrowth and in- creasing amounts of EphB3-Fc protein used for coating onto an L1 substratum. The num- bers in parentheses represent the numbers of explants used in each condition. Data from five independent experiments (mean SEM) are shown. The percentage of retinal ex- plants from EphrinB3 null animals with axon outgrowth on an L1/EphB3-Fc substratum is indicated by the open bar. Data from three independent experiments (mean SEM) are shown. L, Graph showing the dose–response relationship between the amount of out- growth with increasing amounts of EphB3-Fc protein used for coating onto a, L1 substra- tum. The numbers in parentheses represent the total number of explants used in each condition. Data from three independent experiments (mean SEM) are shown. The amount of outgrowth from retinal explants of EphrinB3 null animals on an L1/EphB3-Fc substratum is indicated by the open bar. Data from three independent experiments (mean SEM) are shown. Scale bars: A, B, 50 m; C, D, G, 5 m; E, F, 2 m; H, 100 m. LN, Laminin.

    Journal: Journal of Neuroscience

    Article Title: EphB3: An Endogenous Mediator of Adult Axonal Plasticity and Regrowth after CNS Injury

    doi: 10.1523/jneurosci.4797-05.2006

    Figure Lengend Snippet: Figure 6. EphB3-Fc protein supports adult RGC axon outgrowth. A, Adult retinal ex- plant grown on a substratum coated with low levels of laminin (2.5 g/ml). This level of laminincoatingsupportsonlyminimalaxonoutgrowth(arrowpointstoanaxon).B,Adult retinal explant grown for 3 d on a substratum coated with low laminin (2.5 g/ml) and EphB3-Fc (9 g/ml). The presence of EphB3-Fc increased the amount of axon outgrowth from adult retinal explants. C, Growth cone extending on low laminin (2.5 g/ml) and EphB3-Fc (9 g/ml). D, Growth cone extending on L1 (0.25 g/ml) and EphB3-Fc (10 g/ml).E,Anti-tubulinstaininginagrowthconesimilarto C.F,Phalloidinstaininginthe same growth cone as in E. G, Anti-GAP-43 immunostaining in a retinal axon grown on a laminin substratum (10 g/ml). H, Immunostaining showing L1 protein expression on RGC axons within the optic nerve 12 d after injury. The asterisk indicates the injury site. I, Graph showing the dose–response relationship between the mean axon number with increasing concentrations of EphB3-Fc protein used for coating onto a laminin substra- tum.Thenumbersatthetopofeachcolumnindicatethenumberofexplantsexaminedfor the given experimental condition. J, Graph showing the relationship between total axon length (mean SEM) with increasing concentrations of EphB3-Fc protein used for sub- stratum coating. The numbers at the top of each column indicate the number of explants examined for the given experimental condition. K, Graph showing the dose–response relationship between the percentage of retinal explants with axon outgrowth and in- creasing amounts of EphB3-Fc protein used for coating onto an L1 substratum. The num- bers in parentheses represent the numbers of explants used in each condition. Data from five independent experiments (mean SEM) are shown. The percentage of retinal ex- plants from EphrinB3 null animals with axon outgrowth on an L1/EphB3-Fc substratum is indicated by the open bar. Data from three independent experiments (mean SEM) are shown. L, Graph showing the dose–response relationship between the amount of out- growth with increasing amounts of EphB3-Fc protein used for coating onto a, L1 substra- tum. The numbers in parentheses represent the total number of explants used in each condition. Data from three independent experiments (mean SEM) are shown. The amount of outgrowth from retinal explants of EphrinB3 null animals on an L1/EphB3-Fc substratum is indicated by the open bar. Data from three independent experiments (mean SEM) are shown. Scale bars: A, B, 50 m; C, D, G, 5 m; E, F, 2 m; H, 100 m. LN, Laminin.

    Article Snippet: After transfer to nitrocellulose, blots were blocked with PBS/5% anti-donkey serum or dry milk and probed with anti-mouse EphB3 (1:1000; R & D Systems) or anti-human EphrinB3 (1:500; R & D Systems).

    Techniques: Immunostaining, Expressing